Team:Warsaw/Calendar-Main/19 September 2008

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(Difference between revisions)
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<h3>MutD<sub>5</sub> testing</h3>
<h3>MutD<sub>5</sub> testing</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
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Inoculation MutD<sub>5</sub> carrying: OmpA_A_alpha, OmpA_Z_omega i OmpA_omega_A + induction using 0.25mM iPTG
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Testing MutD<sub>5</sub> ampicylin resistance - MutD<sub>5</sub> has not spontaneous ampicylin resistance mutations.
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Revision as of 11:29, 25 October 2008

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MutD5 testing

Piotr

Testing MutD5 ampicylin resistance - MutD5 has not spontaneous ampicylin resistance mutations.

'Hunter and prey' system tests: Competition tests

Weronika

Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

    • Omp_A_omega, Omp_omega and Omp_Z_omega - A_alpha protein added ~100 μg
    • Omp_A_omega, Omp_omega and Omp_Z_omega - Z_alfa protein added ~100 μg
    • Omp_A_alpha, Omp_alpha and Omp_Z_alpha - Z_omega protein added ~100 μg

  2. Bacteria centrifuged and freez

Preparation of BioBricks

Michał K.

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of RFP(??????) and deltaA.

Piotr

  1. E. coli TOP10 transformation with the above ligation.
  2. Plating on LB + ampicillin.

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