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Team:Chiba/Receiver experiments
From 2008.igem.org
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==LuxR mutants== | ==LuxR mutants== | ||
[[Image:Location-of-the-mutant-in-LuxR Chiba.gif|frame|right|'''Fig.6''' Location of the LuxR mutant in LuxR]] | [[Image:Location-of-the-mutant-in-LuxR Chiba.gif|frame|right|'''Fig.6''' Location of the LuxR mutant in LuxR]] | ||
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私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 | 私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 | ||
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*'''Sender''' | *'''Sender''' | ||
**[http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL auto inucer)] | **[http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL auto inucer)] | ||
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# Measured intensity of green fluorescence at regular time intervals. | # Measured intensity of green fluorescence at regular time intervals. | ||
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Revision as of 11:41, 25 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
|---|
Contents |
Receiver crosstalk
Method
Result
LuxR mutants
Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。
Method
- Sender
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL auto inucer)]
- Receivers
- mutant LuxR Receiver
- WT LuxR Receiver ([http://partsregistry.org/Part:BBa_S03623 BBa_T9002 ])
LuxR mutantsとWT LuxRのディレイタイムはfollowing procedureによってanalyzeした。
- Transformed sender (Ptet-luxI), mutant LuxR Receiver (Ptet-mLuxR-Plux-GFP) and WT LuxR Receiver ()into E coli strains (BW⊿FliC)
- Inoculated Sender, WT Receiver (wild type luxR/BW⊿FliC) and mutated Receiver (1point mutation/BW⊿FliC) in liquid media for 12 h at 37℃.
- Inoculated again in liquid media upto about OD600=2 at 37℃
- Washed Senders and receiver.
- Mixed them. (Sender:Receiver=1000μL:1000μL)
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Result
| Home | The Team | The Project | Parts Submitted to the Registry | Notebook |
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