Team:NTU-Singapore/Notebook/5 July 2008
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**Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results. | **Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results. | ||
**Prepare digestion mixtures for gel confirmation. | **Prepare digestion mixtures for gel confirmation. | ||
- | + | ===Darius=== | |
+ | :Gel electrophoresis to check if there are LacI-RBS 34 plasmids.<br>Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band: | ||
**There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!) | **There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!) | ||
**There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS). | **There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS). | ||
**The rest only have the RBS band. | **The rest only have the RBS band. | ||
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Revision as of 11:57, 25 October 2008
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Contents |
Saturday 5 July
Lu Chao
Hung, Lu Chao
Darius
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