Team:NTU-Singapore/Notebook/14 July 2008

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=Monday 14 July=
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*LacI-GFP COLONIES!!  
*LacI-GFP COLONIES!!  
**Miniprep of 18 colonies picked and one for T7ptag
**Miniprep of 18 colonies picked and one for T7ptag
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**GFP vector also from last Friday digestion.
**GFP vector also from last Friday digestion.
**This time, we used 2 insert:vector ratios (1:3 and 1:6).
**This time, we used 2 insert:vector ratios (1:3 and 1:6).
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Revision as of 12:11, 25 October 2008

Monday 14 July

  • LacI-GFP COLONIES!!
    • Miniprep of 18 colonies picked and one for T7ptag
    • Gel check was ran - 5 colonies had properly ligated LacI-GFP (got 1.1kb band), but T7ptag was not ligated well.

Well Done!

  • Ligation of Fe and GFP was also carried out.
    • pFe insert from last Friday (stored at -20). We also did nanodrops before ligating to measure the concentration.
    • GFP vector also from last Friday digestion.
    • This time, we used 2 insert:vector ratios (1:3 and 1:6).
Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/14_July_2008"