Revision as of 13:03, 25 October 2008

Tuesday 26 August

Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check.
Hung: digestional check:
Results:
- Among 4 samples of LsrA-RBS-Lysis-Term, 1 sample showed the correct bands: 2kb vector + 450bp insert (total length of LsrA+RBS+Lysis+Term). Our detection+lysis system is done!!
- 2 samples of LacI-RBS-E7-Term showed wrong bands. However, ligation mixture for this was still not used up. We would transform the remaining mixture into top10 cells on Friday.

from left to right: 1kb ladder, 2xLacI-RBS-E7-Term, 100bp ladder, LsrA-RBS(for comparing), 4xLsrA-RBS-Lysis-Term, Lysis-Term(for comparing)

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 =Tuesday 26 August=
+<div class="quote" style="margin:20px;">
+{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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 *Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check.
 *Hung: digestional check:<br/>Results:
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 [[Image:NTU_gelcheck_2608.JPG|500px|center|thumb|from left to right: 1kb ladder, 2xLacI-RBS-E7-Term, 100bp ladder, LsrA-RBS(for comparing), 4xLsrA-RBS-Lysis-Term, Lysis-Term(for comparing)]]
+</div>