Team:NTU-Singapore/Notebook/29 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: =Friday 29 August= *Lu Chao: prepared new top10 competent cells (60x50ul tubes). *Hung: transformation into top10 for: **The remaining ligation mixture of LacI-RBS-E7-Term. **LacI-RBS (as ...)
Line 1: Line 1:
 +
<html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html>
 +
 +
<div id="header">{{User:Greenbear/sandbox/header}}</div>
 +
 +
<div id="maincontent" style="margin-top:100px;">
 +
<html>
 +
<div id="arrow">
 +
<a href="https://2008.igem.org/Team:NTU-Singapore/Notebook">
 +
<img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png
 +
"
 +
    alt="Back to Notebook"
 +
    title="Back to Notebook">
 +
</a>
 +
</div>
 +
</html>
 +
 +
=Friday 29 August=
=Friday 29 August=
 +
<div class="quote" style="margin:20px;">
 +
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
 +
|
*Lu Chao: prepared new top10 competent cells (60x50ul tubes).
*Lu Chao: prepared new top10 competent cells (60x50ul tubes).
*Hung: transformation into top10 for:
*Hung: transformation into top10 for:
Line 5: Line 25:
**LacI-RBS (as only 1 tube of LacI-RBS was left)(for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
**LacI-RBS (as only 1 tube of LacI-RBS was left)(for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
**LsrA-RBS-Lysis-Term (our detection system that was done on Tuesday). (for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
**LsrA-RBS-Lysis-Term (our detection system that was done on Tuesday). (for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
 +
</div>

Revision as of 13:05, 25 October 2008


Friday 29 August

  • Lu Chao: prepared new top10 competent cells (60x50ul tubes).
  • Hung: transformation into top10 for:
    • The remaining ligation mixture of LacI-RBS-E7-Term.
    • LacI-RBS (as only 1 tube of LacI-RBS was left)(for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
    • LsrA-RBS-Lysis-Term (our detection system that was done on Tuesday). (for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/29_August_2008"