"
Team:Warsaw/Calendar-Main/27 August 2008
From 2008.igem.org
(Difference between revisions)
PPrzanowski (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 15: | Line 15: | ||
| - | <h3>Cloning of protein A DNA to < | + | <h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> plasmid</h3><h4>Michał K.</h4> |
| - | <ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/ | + | <ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> with NdeI and NotI (Orange buffer), pET15b was also <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylated</a>. </li> |
<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li> | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li> | ||
Revision as of 13:54, 25 October 2008
 |
|
||||||||||||||||||||||||
 |
|
||||||||||||||||||||||||
Tests for ampicillin resistance given by protein added to mediumPiotrResults of growth from previus day
Cloning of protein A DNA to pACYC177+OmpA_Z_alpha plasmidMichał K.
|
|
