Team:Chiba/Sender experiments

From 2008.igem.org

(Difference between revisions)

Revision as of 14:17, 25 October 2008

Home	The Team	The Project	Parts Submitted to the Registry	Notebook

Quorum-Sensing Cross-talk

Purpose

To Confirm that communication using non-endogenous molecules results in slower activation of receivers.

冨永

Fig.5 Senders crosstalk

Senders

[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]

[http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]

[http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]

Receiver

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
Inoculated them independently in liquid media. Incubated at 37°C 12h
Mixed them.
Incubated at 30°C.
Measured intensity of green fluorescence at regular time intervals.

Result

1.Crosstalk test

Fig. senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.

RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった。
LasIは、GFP inductionが他より約2時間遅れた。

2.Demonstration

>Back to the project page

Home	The Team	The Project	Parts Submitted to the Registry	Notebook

@@ Line 40: / Line 40: @@
 ===Result===
-====1.Crosstalk test at 30°C ====
+====1.Crosstalk test====
 [[image:senders_crosstalk_chiba_01.gif|frame|left|
 '''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all">