Team:Warsaw/Calendar-Main/13 October 2008

From 2008.igem.org

(Difference between revisions)
MKrzyszton (Talk | contribs)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3>Preparation of BioBricks</h3> <h4>Michał K.</h4> <ol> <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/p...)
Newer edit →

Revision as of 17:09, 25 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of BioBricks

Michał K.

  1. Digest of 3kb(????)+pLac_OmpA_omega with BcuI and SacI (SacI buffer), dephosphorylation with CIAP.
  2. PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using LinLSXNE and AlfaPSpe primers (annealing temperature 58 °C; elongation length 60s) to obtain link_alpha fragment.
  3. PCR on alpha PCR product from 10 September using AlphaL+SacI and LinP_BS2 primers (annealing temperature 58 °C; elongation length 60s) to obtain alpha fragment with proper restriction sites.




Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/13_October_2008"