Team:Warsaw/Calendar-Main/11 September 2008

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<p><ol><li>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).</li>
<p><ol><li>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).</li>
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<li>Mesurment of growth after 6 hours - 2 fold digger growth with interacting proteins (Omp_A_omega twice as more as Omp_omega with protein Z_alpha etc..)!!!
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<li>Measurment of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (Omp_A_omega twice as more as Omp_omega with protein Z_alpha etc..)</li></ol></p>
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We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>
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<p>We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>

Revision as of 18:16, 25 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).
  2. Measurment of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (Omp_A_omega twice as more as Omp_omega with protein Z_alpha etc..)

We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!

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