"
User:Bipedalape/1 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: <br>PCR: MioC (Promoter Region from JM109) <br>Verification of Antibiotic Plates Potency) |
Bipedalape (Talk | contribs) |
||
| Line 1: | Line 1: | ||
<br>PCR: MioC (Promoter Region from JM109) | <br>PCR: MioC (Promoter Region from JM109) | ||
| - | <br>Verification of Antibiotic Plates Potency | + | |
| + | PCR settings: | ||
| + | 95 3min | ||
| + | {95 0.5 min | ||
| + | 50 0.5 min | ||
| + | 72 1 min) | ||
| + | |||
| + | (Repeated X 30) | ||
| + | |||
| + | 72 10 min | ||
| + | 4 hold | ||
| + | |||
| + | PCR Product of ~300bp (Successful) | ||
| + | w/o DMSO | ||
| + | |||
| + | <br>Verification of Antibiotic Plates Potency: Colonies were isolated on Chloro, Kana, Amp, and Tet. Successful | ||
Revision as of 19:01, 25 October 2008
PCR: MioC (Promoter Region from JM109)
PCR settings: 95 3min {95 0.5 min 50 0.5 min 72 1 min)
(Repeated X 30)
72 10 min 4 hold
PCR Product of ~300bp (Successful) w/o DMSO
Verification of Antibiotic Plates Potency: Colonies were isolated on Chloro, Kana, Amp, and Tet. Successful
