Team:Warsaw/Calendar-Main/29 May 2008
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<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A+AID</a> (in liquid LB) into liquid LB + 1 mM IPTG.</li> | <li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A+AID</a> (in liquid LB) into liquid LB + 1 mM IPTG.</li> | ||
- | <li> | + | <li> Cultures were incubated for 5 hours.</li> |
- | <li> 1 ml (2x) from p.1, p.2, p.3, p.4 | + | <li> 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin. </li> |
</ol></p> | </ol></p> | ||
Revision as of 19:02, 25 October 2008
Rifampicin testMichał L., Ewa, Marcin
PiotrOD measurement of above cultures. |