Team:Warsaw/Calendar-Main/17 October 2008

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.</li>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.</li>
<li>Gel electrophoresis of over night digest (RFP(?????) + pT7_alpha) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band 800 bp. </li>
<li>Gel electrophoresis of over night digest (RFP(?????) + pT7_alpha) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band 800 bp. </li>
-
 
+
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments: 5kb(?????) + pT7_alpha.</li>
</ol>
</ol>
<h3></h3>
<h3></h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
-
<ol><li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with overnight ligation - RFP(?????) + AraC+pBAD+AID.</li>
+
<ol><li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with overnight ligation - RFP(?????) + AraC+pBAD+AID and 5kb(?????) + pT7_alpha.</li>
-
<li> Planting on LB with ampicillin.</li></ol>
+
<li> Planting: RFP(?????) + AraC+pBAD+AID on LB with ampicillin and 5kb(?????) + pT7_alpha on LB with kanamycin.</li></ol>
</html>
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 20:11, 25 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of BioBricks

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_omega_.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.
  3. Gel electrophoresis of over night digest (RFP(?????) + pT7_alpha) and gel-out of proper band 800 bp.
  4. Ligation of isolated DNA fragments: 5kb(?????) + pT7_alpha.

Piotr

  1. Transformation of TOP10 with overnight ligation - RFP(?????) + AraC+pBAD+AID and 5kb(?????) + pT7_alpha.
  2. Planting: RFP(?????) + AraC+pBAD+AID on LB with ampicillin and 5kb(?????) + pT7_alpha on LB with kanamycin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/17_October_2008"