Team:Warsaw/Calendar-Main/29 August 2008

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Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

Isolation of pET15b+A-alpha plasmids.
Control digest of isolated plasmids with XbaI and BamHI (Tango buffer).
Gel electrophoresis. Choice of proper clone.

Protein purification: A-alpha

Piotr

Single transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin.

Blue-white screening and rifampicin test in GM2163

Michał L.

GM2163 competent cells carrying pZC320 were transformed with following plasmids:

Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant.

@@ Line 5: / Line 5: @@
 <p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a> plasmids.</li>
 <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with XbaI and BamHI (Tango buffer). </li>
-<li>Gel elctrophoresis. Chioce of proper clone. </li>
+<li>Gel electrophoresis. Choice of proper clone. </li>
 </ol></p>
@@ Line 18: / Line 18: @@
 <h4>Michał L.</h4>
-<p>GM2163 competent cells carrying pZC320 were transformed with following plasmids: </p>
+<p>GM2163 competent cells carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> were transformed with following plasmids: </p>
 <ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5-omega</a>,</li>