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Team:Montreal/DNA Agarose Gel
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(New page: 1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder. 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling...)
Newer edit →
(New page: 1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder. 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling...)
Newer edit →
Revision as of 22:50, 16 June 2008
1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder.
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).
3. Add 1.5µL of Ethimium Bromide and swirl until a fine mixture is seen.
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.
5. Wait for the gel to solidify over time.
6. Once hardened, remove the comb and load the gel into the electrophoresis box.
