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Team:Montreal/DNA Agarose Gel
From 2008.igem.org
(Difference between revisions)
(New page: 1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder. 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling...) |
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| - | 1. | + | 1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder. |
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling). | 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling). | ||
| - | 3. Add 1.5µL of | + | 3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen. |
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends. | 4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends. | ||
Revision as of 22:51, 16 June 2008
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).
3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen.
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.
5. Wait for the gel to solidify over time.
6. Once hardened, remove the comb and load the gel into the electrophoresis box.
