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Team:Warsaw/Calendar-Main/6 May 2008
From 2008.igem.org
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<li>Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics.</li></ol><br> | <li>Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics.</li></ol><br> | ||
| - | <h3>Isolation of genomic DNA</h3> | + | <h3> Isolation of genomic DNA </h3> |
<h4>Michał L.</h4> | <h4>Michał L.</h4> | ||
Revision as of 00:57, 26 October 2008
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Preparation of pMPMT5+AID constructPiotr, Weronika
Isolation of genomic DNAMichał L.
Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control for PCR. Fig. 1. 
Fig. 1. Probes of isolated genomic DNA after mechanical shearing by pipetting. Without shearing the DNA wouldn't be visible in this kind of gel because genomic DNA is too big. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.
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