Team:Tokyo Tech/Result

From 2008.igem.org

(Difference between revisions)

Revision as of 05:20, 26 October 2008

Home	Construction	Acrylic container	Development of promoter	Genetic toggle switch	Parts Submitted to the Registry	Result	The Team	Notebook

Checking pressure response ability of Plac

We examined changing of GFP fluorescence strength with pressurizing.At the same time, we examined fluorescence strength by adding IPTG under 0.1MPa.As a way of observation, we adopted a fluorescence microscope, FACS and FLA.FACS and FLA is for quantitative analysis of fluorescence strength. FLA’s result is Fig2, Fig3, Fig4.

Fig 2: Average of Plac /p>

　Fig 3: Average of ⊿p

Fig 2: Average of Plac /p>

　Fig 3: Average of ⊿p

　Fig 4: Average of Ptet

Fig 5: Fig2&3&4 gathers into one chart.

Figure 2, 3, 4 ; These charts show change of Geo Mean under each pressures. Figure 2 is about Plac-GFP. We constructed Plac-GFP. E. coli strains is JM109. Between 0.1MPa and 30MPa, Geo Mean almost don’t change.But at 50MPa Geo Mean increase sharply. Figure 3 is about promoter less-GFP (∆p-GFP) as a negative control. Between 0.1MPa and 30MPa, Geo Mean almost don’t change. But, at 50MPa Geo Mean increase sharply. Figure 4, is about Ptet-GFP as a positive control.Increasing pressure, Geo Mean increases.

A fluorescence microscope is for qualitative analysis of fluorescence strength. Fluorescence microscope image is Fig 6.

@@ Line 26: / Line 26: @@
          </tr>
        </table>
-<br><br>
 <table width="100%" border="0" cellspacing="0" cellpadding="0">
          <tr>