Team:Tokyo Tech/Construction

From 2008.igem.org

(Difference between revisions)

Revision as of 06:02, 26 October 2008

Home	Construction	Acrylic container	Development of promoter	Genetic toggle switch	Parts Submitted to the Registry	Result	The Team	Notebook

Construction

For confirming pressure-response ability of pressure-inducible promoter, we experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_I739201 BBa_I739201]), the other is promoter less-GFP on pSB6.

@@ Line 57: / Line 57: @@
 For confirming pressure-response ability of pressure-inducible promoter, we
-experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet on pSB6([http://partsregistry.org/Part:BBa_I739201 BBa_I739201]), the other is ∆p on pSB6.
+experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_I739201 BBa_I739201]), the other is promoter less-GFP on pSB6.
-Ptet stands for promoter repressed by tetR protein. ∆p stands for promoter less. JM109 is used .And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control.