Team:Tokyo Tech/Construction

From 2008.igem.org

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For confirming pressure-response ability of pressure-inducible promoter, we  
For confirming pressure-response ability of pressure-inducible promoter, we  
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experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_I739201 BBa_I739201]), the other is promoter less-GFP on pSB6.
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experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6(http://partsregistry.org/Part:BBa_K121013 BBa_K121013).

Revision as of 06:04, 26 October 2008

Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Notebook

Construction

For confirming pressure-response ability of pressure-inducible promoter, we experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6(http://partsregistry.org/Part:BBa_K121013 BBa_K121013).