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Team:Tokyo Tech/Construction
From 2008.igem.org
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For confirming pressure-response ability of pressure-inducible promoter, we | For confirming pressure-response ability of pressure-inducible promoter, we | ||
experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]). | experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]). | ||
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| + | [[Image:Ptet-GFP.png|250px|center|fig1.Ptet-GFP]] | ||
| + | [[Image:promoter_less-GFP.png|180px|center|fig1.Promoter less-GFP]] | ||
Revision as of 06:17, 26 October 2008
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Construction
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]).
