Team:Tokyo Tech/Construction

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Revision as of 07:26, 26 October 2008

Home	Construction	Acrylic container	Development of promoter	Genetic toggle switch	Parts Submitted to the Registry	Result	The Team	Notebook

Construction

figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment

For confirming pressure-response ability of pressure-inducible promoter, we experimented under several pressures. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]).

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 [[Image:Construction.png|260px|thumb|left|figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment]]
-We experimented under several pressures so as to confirm pressure-response ability of TetR promoter(PtetR) which we chose as pressure-inducible promoter.
+For confirming pressure-response ability of pressure-inducible promoter, we experimented under several pressures. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]).
-we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]).