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Team:Tokyo Tech/Construction
From 2008.igem.org
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| - | == ''' | + | == '''Confirmatory experiment''' == |
[[Image:Construction.png|260px|thumb|left|figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment]] | [[Image:Construction.png|260px|thumb|left|figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment]] | ||
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 0.1MPa and 30MPa pressure. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]) as a negative control. | For confirming pressure-response ability of pressure-inducible promoter, we experimented under 0.1MPa and 30MPa pressure. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]) as a negative control. | ||
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[[Image:Pressure-response_ability_of_PtetR.png|350px|thumb|right|figure2 Result of experiment]] | [[Image:Pressure-response_ability_of_PtetR.png|350px|thumb|right|figure2 Result of experiment]] | ||
| - | The result | + | |
| + | The result was that PtetR activity under 30MPa pressure is 2.5 times stronger than PtetR activity under 0.1MPa pressure. | ||
Revision as of 08:17, 26 October 2008
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Confirmatory experiment
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 0.1MPa and 30MPa pressure. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]) as a negative control.
The result was that PtetR activity under 30MPa pressure is 2.5 times stronger than PtetR activity under 0.1MPa pressure.
