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Team:Tokyo Tech/Construction
From 2008.igem.org
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[[Image:Pressure-response_ability_of_PtetR.png|350px|thumb|right|figure2 Result of experiment]] | [[Image:Pressure-response_ability_of_PtetR.png|350px|thumb|right|figure2 Result of experiment]] | ||
| - | The result was that PtetR activity under 30MPa pressure is 2.5 times stronger than PtetR activity under 0.1MPa pressure. | + | The result was that PtetR activity under 30MPa pressure is about 2.5 times stronger than PtetR activity under 0.1MPa pressure. Therefore, we confirmed that PtetR was induced under 30MPa pressure. |
Revision as of 09:44, 26 October 2008
| Home | Construction | Acrylic container | Development of promoter | Genetic toggle switch | Parts Submitted to the Registry | Result | The Team | Acknowledgements |
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Confirmatory experiment
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 0.1MPa and 30MPa pressure. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]) as a negative control.
The result was that PtetR activity under 30MPa pressure is about 2.5 times stronger than PtetR activity under 0.1MPa pressure. Therefore, we confirmed that PtetR was induced under 30MPa pressure.
