"
Team:Chiba/Calendar-Home/16 September 2008
From 2008.igem.org
(Difference between revisions)
(→Team:Communication) |
(→Team:Communication) |
||
| Line 6: | Line 6: | ||
===Team:Communication=== | ===Team:Communication=== | ||
| - | + | *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] with (15/9) Transformants. | |
| - | + | (reaction temparaturewas 37°C and 30°C.) | |
===Team:Output=== | ===Team:Output=== | ||
Revision as of 12:34, 26 October 2008
15 September 2008 <|> 17 September 2008
Laboratory work
Team:Communication
- time-delay test with (15/9) Transformants.
(reaction temparaturewas 37°C and 30°C.)
Team:Output
Digestion test
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
- [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
| Sample No. | each |
| DNA(μL) | 3 |
| EcoRⅠ(μL) | 0.083 |
| PstⅠ(μL) | 0.083 |
| BSA(x10)(μL) | 1 |
| Buffer3(x10)(μL) | 1 |
| dH2O(μL) | 5 |
| TOTAL(μL) | 10 |
-->37℃ 1hour
| Sample No. | each |
| DNA(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
- [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
- [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
- [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
- [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)
- Venus YFP(ptet+LuxR+YFP)
| Sample No. | YFP |
| DNA(μL) | 1 |
| Venus Fwd(μL) | 5 |
| Rev(VR)(μL) | 5 |
| Thermo pol Buffer(μL) | 10 |
| dNTP mix(μL) | 10 |
| Vent(μL) | 1 |
| dH2O(μL) | 68 |
| TOTAL(μL) | 100 |
