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| - | == <font size=5> Checking pressure response ability of Plac </font> == | + | == Protocol == |
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| - | <p class=MsoNormal>We examined changing of GFP fluorescence strength with pressurizing.At the same time, we examined fluorescence strength by adding IPTG under 0.1MPa.As a way of observation, we adopted a fluorescence microscope, FACS and FLA.FACS and FLA is for quantitative analysis of fluorescence strength. FLA’s result is Fig2, Fig3, Fig4.</font></p>
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| - | <p class=MsoNormal>Fig 2: Average of Plac /p></td>
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| - | <td class="a" width="40%"> Fig 3: Average of ⊿p </td>
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| - | <p class=MsoNormal>Fig 2: Average of Plac /p></td>
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| - | <td class="a" width="40%"> Fig 3: Average of ⊿p </td>
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| - | <td class="a"> Fig 4: Average of Ptet</td>
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| - | <td class="a" width="40%"> Fig 5: Fig2&3&4 gathers into one chart.</td>
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| - | <p class=MsoNormal>Figure 2, 3, 4 ; These charts show change of Geo Mean under each pressures. Figure 2 is about Plac-GFP. We constructed Plac-GFP. E. coli strains is JM109. Between 0.1MPa and 30MPa, Geo Mean almost don’t change.But at 50MPa Geo Mean increase sharply. Figure 3 is about promoter less-GFP (∆p-GFP) as a negative control. Between 0.1MPa and 30MPa, Geo Mean almost don’t change. But, at 50MPa Geo Mean increase sharply. Figure 4, is about Ptet-GFP as a positive control.Increasing pressure, Geo Mean increases. </p></td>
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| - | A fluorescence microscope is for qualitative analysis of fluorescence strength. Fluorescence microscope image is Fig 6.</td>
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