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Team:Paris/Modeling/f5
From 2008.igem.org
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| - | [[Image:f5DCA.png|thumb|[Team:Paris/Modeling/Protocol_Of_Characterisation|Characterisation Plasmid]]] | + | [[Image:f5DCA.png|thumb|[[Team:Paris/Modeling/Protocol_Of_Characterisation|Characterisation Plasmid]] ]] |
We have [FlhDC] = {coef<sub>flhDC</sub>}''expr(pTet)'' = {coef<sub>flhDC</sub>} ƒ1([aTc]<sub>i</sub>) | We have [FlhDC] = {coef<sub>flhDC</sub>}''expr(pTet)'' = {coef<sub>flhDC</sub>} ƒ1([aTc]<sub>i</sub>) | ||
Revision as of 14:44, 26 October 2008
We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)
and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)
So, at steady-states,
| param | signification | unit | value | comments |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.min-1 | need for 20 mesures with well choosen values of [aTc]i and for 20 mesures with well choosen values of [arab]i and 5x5 measures for the relation below? | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the relation below? | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the relation below? | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| β52 | production rate of FlhDC-pFliL with RBS E0032 β52 | nM.min-1 | ||
| (K46/{coeffliA}) | activation constant of FlhDC-pFliL K46 | nM | ||
| n46 | complexation order of FlhDC-pFliL n46 | no dimension | ||
| β53 | production rate of FliA-pFliL with RBS E0032 β53 | nM.min-1 | ||
| (K47/{coefomp}) | activation constant of FliA-pFliL K47 | nM | ||
| n47 | complexation order of FliA-pFliL n47 | no dimension |
Then, if we have time, we want to verify the expected relation
