Team:Warsaw/Calendar-Main/9 September 2008

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<h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4>  
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<h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4>  
<p>Measurement of concentration of protein(<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">BCA method</a>). </p>
<p>Measurement of concentration of protein(<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">BCA method</a>). </p>

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Purification of proteins: A-alpha

Piotr

Measurement of concentration of protein(BCA method).

Tests for ampicillin resistance given by protein added to medium

Piotr

Inoculation of TOP10 carrying pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega to LB with inducer (0.25 mM IPTG) and kanamycin.

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