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Team:Warsaw/Calendar-Main/7 August 2008
From 2008.igem.org
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-deltaA>pACYC177+omega_ΔA</a>) with HindIII and SacI (BamHI buffer)(Fig. 1.). 7 from 8 colonies confirmed. </li></ol></p> | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-deltaA>pACYC177+omega_ΔA</a>) with HindIII and SacI (BamHI buffer)(Fig. 1.). 7 from 8 colonies confirmed. </li></ol></p> | ||
| - | <h3>Checking | + | <h3>Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>)</h3> |
<h4>Michał L.</h4> | <h4>Michał L.</h4> | ||
<p>Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin.</p> | <p>Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin.</p> | ||
Revision as of 17:42, 26 October 2008
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Purification of proteins: Z-alpha and Z-omegaPiotr, EmiliapET15b+Z-alpha and pET15b+Z-omega inoculated for overnight culture (Rosetta strain) Preparing of pACYC177+OmpA_omega_ΔA constructMichał K.
Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)Michał L.Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin.  Fig. 1. Control SacI/HindIII digests of isolated plasmids1. Marker 2-9. digested plasmids pACYC177+OmpA_omega_deltaA
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