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Team:Warsaw/Calendar-Main/11 August 2008
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| - | <h3>Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>)</h3> | + | <h3>Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>) <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_August_2008#fig1">Fig. 1</a></h3> |
<h4>Piotr</h4> | <h4>Piotr</h4> | ||
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| - | <img src="https://static.igem.org/mediawiki/2008/7/77/Western2_WAW.jpg" width=400/> | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/77/Western2_WAW.jpg" width=400/></a> |
| - | <var>Detection of protein A. Dalszy opis również wstawię jak się wyśpię...</var> | + | <var>Detection of protein A. Mammoths were here.<Dalszy opis również wstawię jak się wyśpię...></var> |
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Revision as of 17:49, 26 October 2008
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Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10) Fig. 1PiotrTest was conducted in E.coli Rosetta strain expressing OmpA_omega_ΔA_alpha (with and without induction) and OmpA_ΔA_alpha.
Detection of protein A. Mammoths were here.We didn't observe differences in expression and degradation in Rosettas nor in TOP10. Therefore we suppose that degradation of the fusions is caused by factor other than Lon and OmpT proteases. Purification of proteins: Z-alpha and Z-omegaEmilia100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture in presence of ampicillin, kanamycin and IPTG (according to protocol).
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