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Team:Warsaw/Calendar-Main/7 August 2008
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<p>Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin.</p> | <p>Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin.</p> | ||
| - | <h3>Preparing | + | <h3>Preparing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-deltaA>pACYC177+OmpA_omega_ΔA</a> construct</h3><h4>Michał K.</h4> |
<p><ol> | <p><ol> | ||
<li>Isolation of plasmids from cultures inoculated on previous day.</li> | <li>Isolation of plasmids from cultures inoculated on previous day.</li> | ||
Latest revision as of 18:35, 26 October 2008
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Purification of proteins: Z-alpha and Z-omegaPiotr, EmiliapET15b+Z-alpha and pET15b+Z-omega inoculated for overnight culture (Rosetta strain). Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)Michał L.Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin. Preparing pACYC177+OmpA_omega_ΔA constructMichał K.
 Fig. 1. Control SacI/HindIII digests of isolated plasmids1. Marker 2-9. digested plasmids pACYC177+OmpA_omega_deltaA
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