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Team:Warsaw/Calendar-Main/14 August 2008
From 2008.igem.org
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<li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands 4400 bp (for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>) and 4200 bp (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>). </li></ol> | <li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands 4400 bp (for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>) and 4200 bp (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>). </li></ol> | ||
| - | <h3>Purification of proteins: | + | <h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Piotr</h4> |
<p>Electrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusios: Z-alpha and Z-omega (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008#fig1">Fig. 1</a>, <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008#fig2">Fig. 2</a>)</p><br> | <p>Electrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusios: Z-alpha and Z-omega (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008#fig1">Fig. 1</a>, <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008#fig2">Fig. 2</a>)</p><br> | ||
Revision as of 18:41, 26 October 2008
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Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Purification of proteins: Z-alpha and Z-omegaPiotrElectrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusios: Z-alpha and Z-omega (Fig. 1, Fig. 2)  Fig. 1. Fractions from purifying Z-alpha and Z-omega protein fusions.1. purified Z-omega 2. supernatant Z-alpha after sonication 3. elution of Z-alpha using ddH2o 4. purified Z-alpha 5. Marker  Fig. 2. Sonicate and pellet from Z-omega induction1. Marker 2. sonicate Z-omega after induction 3. pellet Z-omega after induction
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