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Team:Chiba/Receiver
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==Quorum_Sensing_Crosstalk== | ==Quorum_Sensing_Crosstalk== | ||
===Design=== | ===Design=== | ||
| + | [[Image:Table R chiba.gif|frame|left|Table.1 LuxR family gene]] | ||
| + | センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山） | ||
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===Experiment=== | ===Experiment=== | ||
==Plasmid_Copy_number== | ==Plasmid_Copy_number== | ||
Revision as of 18:56, 26 October 2008
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Signal Molecule Receiver
Quorum_Sensing_Crosstalk
Design
Table.1 LuxR family gene
センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）
Experiment
Plasmid_Copy_number
Design
レシーバーのコピーナンバーを減らすことで、thresholdに達するまでの時間を伸ばす。 （小林）
Experiment
LuxR_mutant
Design
Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)
Experiment
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