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Team:Heidelberg/Notebook/Sensing Group/Notebook/8thweek
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== Monday, 09/22/2008 == | == Monday, 09/22/2008 == | ||
| + | * test digestion of F1_LuxP_NdeI (F1 cloned in pDK48 with NdeI), F2_LuxP_NdeI, F1_LuxP_KpnI (F1 cloned in pDK48 with KpnI) and F2_LuxP_KpnI (F2 cloned in pDK48 with KpnI) with NcoI | ||
| + | [[Image:HD 080922 Fusion LuxP in pDK48.png|center|thumb|400px|Digestions of Fusion constructs with NcoI. Positive clones show two bands at 4308 and 2915 bp]] | ||
| + | * Transformation of Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48) into MG1655 and HCB33 cells | ||
| + | * Preparation of cultures for Maxipreps of Fusion constructs | ||
== Tuesday, 09/23/2008 == | == Tuesday, 09/23/2008 == | ||
Revision as of 19:07, 26 October 2008
Contents |
Monday, 09/22/2008
- test digestion of F1_LuxP_NdeI (F1 cloned in pDK48 with NdeI), F2_LuxP_NdeI, F1_LuxP_KpnI (F1 cloned in pDK48 with KpnI) and F2_LuxP_KpnI (F2 cloned in pDK48 with KpnI) with NcoI
- Transformation of Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48) into MG1655 and HCB33 cells
- Preparation of cultures for Maxipreps of Fusion constructs
