Team:Warsaw/Calendar-Main/19 August 2008
From 2008.igem.org
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligations products.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligations products.</li> | ||
- | <li> Transformants plating on LB + | + | <li> Transformants plating on LB + kanamycin.</li></ol> |
Revision as of 20:45, 26 October 2008
Tests for ampicillin resistance given by protein added to mediumPiotrInoculation of TOP10 carrying pACYC177+OmpA_A_alpha, pACYC177+OmpA_ΔA_alpha, pACYC177+OmpA_ΔA_omega, pACYC177+OmpA_A_omega, pACYC177+OmpA_omega_ΔA, pACYC177+OmpA_alpha, pACYC177+OmpA_omega and pACYC177+OmpA_omega_ΔA_alpha to LB with inductor (0.25 mM IPTG) and kanamycin. Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Cloning of protein A DNA to pET15b+OmpA-alpha plasmidAntoni
Cloning of protein A DNA to GeneArt plasmidAntoni
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