Team:Warsaw/Calendar-Main/22 August 2008
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<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4><ol> | <h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4><ol> | ||
<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a> plasmids with NdeI and NotI (Orange buffer). </li> | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a> plasmids with NdeI and NotI (Orange buffer). </li> | ||
- | <li>Gel | + | <li>Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008#fig1">Fig. 1.</a>). Choice of proper clone. </li></ol> |
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<a name=fig1><img src="https://static.igem.org/mediawiki/2008/d/d0/PGA%2BAtraw.jpg"></a> | <a name=fig1><img src="https://static.igem.org/mediawiki/2008/d/d0/PGA%2BAtraw.jpg"></a> | ||
- | <var><b>Fig. 1.</b>Control digest of freshly ligated pKS+A ( | + | <var><b>Fig. 1.</b>Control digest of freshly ligated pKS+A (lanes 1-3). Lane 4 - 0.5μg of DNA ladder mix. </var> |
<h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Antoni</h4> | <h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Antoni</h4> |
Revision as of 20:49, 26 October 2008
Tests for ampicillin resistance given by protein added to mediumPiotrPurification of pACYC177+OmpA_alpha from antibiotic resistance contamination: Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Cloning of protein A DNA to GeneArt plasmidAntoni
Fig. 1.Control digest of freshly ligated pKS+A (lanes 1-3). Lane 4 - 0.5μg of DNA ladder mix. Cloning of protein A DNA to pET15b+OmpA-alpha plasmidAntoni
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