Team:Warsaw/Calendar-Main/25 August 2008
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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | ||
<p> <ol><li>Purification of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> from antibiotic resistance contamination:<br> | <p> <ol><li>Purification of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> from antibiotic resistance contamination:<br> | ||
- | Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> | + | Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying pACTC177+OmpA_alpha to liquid LB with kanamycin and with kanamycin and ampicillin.</li> |
<li>Inoculation of TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA-alpha</a> to LB with inductor (0.25 mM IPTG) and kanamycin.</p> | <li>Inoculation of TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA-alpha</a> to LB with inductor (0.25 mM IPTG) and kanamycin.</p> | ||
Latest revision as of 20:53, 26 October 2008
Tests for ampicillin resistance given by protein added to mediumPiotr
Cloning of protein A DNA to pET15b+OmpA-alpha plasmidAntoni
Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) were inoculated to liquid LB with kanamycin.
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