Team:Warsaw/Calendar-Main/26 August 2008
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (both potential constructs). </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (both potential constructs). </li> | ||
<li> Control | <li> Control | ||
- | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with FastBamHI and FastAcc65I (Fast Digest buffer).</li> <li> Gel electrophoresis - still no proper clones | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with FastBamHI and FastAcc65I (Fast Digest buffer).</li> <li> Gel electrophoresis - still no proper clones found.</li> |
</ol></p> | </ol></p> | ||
Latest revision as of 20:56, 26 October 2008
Tests for ampicillin resistance given by protein added to mediumPiotr
Cloning of protein A DNA to pET15b+OmpA-alpha plasmidAntoni
Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
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