Team:Harvard/Dailybook/Week7/Widgetry

From 2008.igem.org

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(Co-culture experiment setup)
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Revision as of 21:56, 26 October 2008

Contents

Goals for this week

  • Shewanella and E. coli co-culture experiments
    • If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
    • Motivation:
      • An inducible system in E. coli could be used to control Shewanella current production

Monday: August 4, 2008

Co-culture experiment setup

  • Chambers 1 & 2: wt E. coli + wt Shewie + lactose
  • Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose

Positive Controls

  • Chamber 3: wt E. coli + wt Shewie + lactate
  • Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate

Negative Controls

  • Chamber 7: wt Shewie + lactose
  • Chamber 8: wt E. coli + lactose
  • Chamber 9: Lac-operon knockout E. coli + lactose

Tuesday: August 5, 2008

Co-culture experiment results, day 1

Wednesday: August 6, 2008

Thursday: August 7, 2008

Friday: August 8, 2008