Team:Harvard/Dailybook/Week7/Widgetry
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Linchinlee (Talk | contribs) (→Co-culture experiment setup) |
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=Wednesday: August 6, 2008= | =Wednesday: August 6, 2008= | ||
=Thursday: August 7, 2008= | =Thursday: August 7, 2008= | ||
=Friday: August 8, 2008= | =Friday: August 8, 2008= |
Revision as of 21:56, 26 October 2008
Contents |
Goals for this week
- Shewanella and E. coli co-culture experiments
- If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
- Motivation:
- An inducible system in E. coli could be used to control Shewanella current production
Monday: August 4, 2008
Co-culture experiment setup
- Chambers 1 & 2: wt E. coli + wt Shewie + lactose
- Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose
Positive Controls
- Chamber 3: wt E. coli + wt Shewie + lactate
- Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate
Negative Controls
- Chamber 7: wt Shewie + lactose
- Chamber 8: wt E. coli + lactose
- Chamber 9: Lac-operon knockout E. coli + lactose