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Team:Harvard/Dailybook/Week7/Widgetry
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==Co-culture experiment results, day 1== | ==Co-culture experiment results, day 1== | ||
| - | [[Image: Co-culture_part1.JPG | 700 px]] | + | :[[Image: Co-culture_part1.JPG | 700 px]] |
| + | :* Notes: | ||
| + | :** At t = 2000 s, all cells added | ||
| + | :** At t = 80000 s, all injection done | ||
| - | [[Image: Co-culture_part1_zoomed.jpg | 700 px]] | + | |
| + | === Zoomed-in graph of results, part 1 === | ||
| + | :[[Image: Co-culture_part1_zoomed.jpg | 700 px]] | ||
| + | :* Notes: | ||
| + | :** Zoomed-in on time since injections | ||
| + | :** Positive controls omitted | ||
| + | |||
| + | |||
| + | '''Preliminary Conclusions''' | ||
| + | :* Noticeable difference in behavior between wt E. coli + Shewie and Lac-operon knockout + Shewie co-cultures | ||
| + | :* Positive and negative controls show most likely no other variables involved | ||
| + | :* Could be used to create tic-tac-toe which uses the difference in behavior to determine moves | ||
=Wednesday: August 6, 2008= | =Wednesday: August 6, 2008= | ||
=Thursday: August 7, 2008= | =Thursday: August 7, 2008= | ||
=Friday: August 8, 2008= | =Friday: August 8, 2008= | ||
Revision as of 22:09, 26 October 2008
Contents |
Goals for this week
- Shewanella and E. coli co-culture experiments
- If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
- Motivation:
- An inducible system in E. coli could be used to control Shewanella current production
Monday: August 4, 2008
Co-culture experiment setup
- Chambers 1 & 2: wt E. coli + wt Shewie + lactose
- Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose
Positive Controls
- Chamber 3: wt E. coli + wt Shewie + lactate
- Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate
Negative Controls
- Chamber 7: wt Shewie + lactose
- Chamber 8: wt E. coli + lactose
- Chamber 9: Lac-operon knockout E. coli + lactose
Tuesday: August 5, 2008
Co-culture experiment results, day 1
- Notes:
- At t = 2000 s, all cells added
- At t = 80000 s, all injection done
- Notes:
Zoomed-in graph of results, part 1
- Notes:
- Zoomed-in on time since injections
- Positive controls omitted
- Notes:
Preliminary Conclusions
- Noticeable difference in behavior between wt E. coli + Shewie and Lac-operon knockout + Shewie co-cultures
- Positive and negative controls show most likely no other variables involved
- Could be used to create tic-tac-toe which uses the difference in behavior to determine moves
