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Team:Harvard/Dailybook/Week7/Widgetry
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=== wt E. coli vs. Lac-operon knockout E. coli === | === wt E. coli vs. Lac-operon knockout E. coli === | ||
: [[Image: Co-culture_part2_test.jpg | 700px]] | : [[Image: Co-culture_part2_test.jpg | 700px]] | ||
| - | :* Notes | + | :* Notes: |
:** At t = 260000 s, adjusted gas for Chamber 5 | :** At t = 260000 s, adjusted gas for Chamber 5 | ||
:** At t = 340000 s, lactose injection for Chamber 1 | :** At t = 340000 s, lactose injection for Chamber 1 | ||
:** Initially, very noticeable difference between wt E. coli and Lac-operon knockout E. coli, but the current production of one of the E. coli chambers rises after a day, making the difference less distinguishable | :** Initially, very noticeable difference between wt E. coli and Lac-operon knockout E. coli, but the current production of one of the E. coli chambers rises after a day, making the difference less distinguishable | ||
| - | |||
=== Results w/ positive controls === | === Results w/ positive controls === | ||
Revision as of 22:26, 26 October 2008
Contents |
Goals for this week
- Shewanella and E. coli co-culture experiments
- If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
- Motivation:
- An inducible system in E. coli could be used to control Shewanella current production
Monday: August 4, 2008
Co-culture experiment setup
- Chambers 1 & 2: wt E. coli + wt Shewie + lactose
- Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose
Positive Controls
- Chamber 3: wt E. coli + wt Shewie + lactate
- Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate
Negative Controls
- Chamber 7: wt Shewie + lactose
- Chamber 8: wt E. coli + lactose
- Chamber 9: Lac-operon knockout E. coli + lactose
Tuesday: August 5, 2008
Co-culture experiment results, day 1
- Notes:
- At t = 2000 s, all cells added
- At t = 80000 s, all injection done
- Notes:
Zoomed-in graph of results, part 1
- Notes:
- Zoomed-in on time since injections
- Positive controls omitted
- Notes:
Preliminary Conclusions
- Noticeable difference in behavior between wt E. coli + Shewie and Lac-operon knockout + Shewie co-cultures
- Positive and negative controls show most likely no other variables involved
- Could be used to create tic-tac-toe which uses the difference in behavior to determine moves
Wednesday: August 6, 2008
Co-culture experiment results, day 1 & 2
wt E. coli vs. Lac-operon knockout E. coli
-
- Notes:
- At t = 260000 s, adjusted gas for Chamber 5
- At t = 340000 s, lactose injection for Chamber 1
- Initially, very noticeable difference between wt E. coli and Lac-operon knockout E. coli, but the current production of one of the E. coli chambers rises after a day, making the difference less distinguishable
- Notes:
Results w/ positive controls
- Notes
- At t = 340000 s, lactose injections for Chambers 1, 3, and 6
- Wanted to see if the positive controls would exhibit the same behavior when lactose injected instead of lactate
