Team:Warsaw/Calendar-Main/18 September 2008
From 2008.igem.org
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5. 70 °C link_alpha<br></var> | 5. 70 °C link_alpha<br></var> | ||
- | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09 | + | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width=300/></a><var><b>Fig. 2. PCR to obtain inserts</b><br> |
1. Marker<br> | 1. Marker<br> | ||
2. deltaA<br> | 2. deltaA<br> |
Revision as of 22:35, 26 October 2008
'Hunter and prey' system tests: Competition testsWeronikaInoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_alpha + induction using 0.25mM iPTG MutD5 testingPiotrInoculation of mutD5 into 100 ml of LB and rendering them chemocompetent. Electroporation with pACYC177+OmpA_Z_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_omega_ΔA. Optimisation of primers for preparation of partsMichał K.
Preparation of pSB1A3 carrying ΔA (BBa_K103003)Michał K.
Preparation of pSB2K3 + _alphaMichał K.
Preparation of pSB2K3 + _omegaMichał K.
Preparation of BioBricksMichał K.
1. Marker 2. 55 °C link_alpha 3. 60 °C link_alpha 4. 65 °C link_alpha 5. 70 °C link_alpha Fig. 2. PCR to obtain inserts 1. Marker 2. deltaA 3 omega 4. ompA_omega
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