Team:Rice University/Notebook/17 June 2008
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(New page: =Tuesday 17 June= *Selim Sheikh: **Prepared PCR of lambda DNA (2ul): 50 ul system ***2.5 ul of each of the designed primers STF L (forward) and STF R (reverse) ***1 ul of dNTP ***5 ul of 1...)
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(New page: =Tuesday 17 June= *Selim Sheikh: **Prepared PCR of lambda DNA (2ul): 50 ul system ***2.5 ul of each of the designed primers STF L (forward) and STF R (reverse) ***1 ul of dNTP ***5 ul of 1...)
Newer edit →
Revision as of 19:50, 18 June 2008
Tuesday 17 June
- Selim Sheikh:
- Prepared PCR of lambda DNA (2ul): 50 ul system
- 2.5 ul of each of the designed primers STF L (forward) and STF R (reverse)
- 1 ul of dNTP
- 5 ul of 10X Pfu Buffer
- 0.5 ul of Pfu Ultra
- 36.5 ul of dH2O
- Prepared PCR of lambda DNA (2ul): 50 ul system