Team:Warsaw/Calendar-Main/29 September 2008
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegLNde">OmegLNde</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinP_BS">LinP_BS</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegLNde">OmegLNde</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinP_BS">LinP_BS</a> | ||
primers (annealing temperature 55 °C; elongation length 60s) to obtain omega_link fragment. </li> | primers (annealing temperature 55 °C; elongation length 60s) to obtain omega_link fragment. </li> | ||
- | <li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (ΔA - 250 bp, pLac_OmpA_omega_ - 1200 bp, omega_linker - 350 bp and OmpA_omega_ - 900 bp).</li> | + | <li> Gel electrophoresis of PCR products <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008#fig1">Fig. 1</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (ΔA - 250 bp, pLac_OmpA_omega_ - 1200 bp, omega_linker - 350 bp and OmpA_omega_ - 900 bp).</li> |
</ol> | </ol> | ||
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
<p>Inoculation of bacteria received from iGEM HQs, carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> and <a href=http://partsregistry.org/Part:BBa_I739204>BBa_I739204 (pACYC177 converted into BioBrick vector)</a> plasmids.</p> | <p>Inoculation of bacteria received from iGEM HQs, carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> and <a href=http://partsregistry.org/Part:BBa_I739204>BBa_I739204 (pACYC177 converted into BioBrick vector)</a> plasmids.</p> | ||
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+ | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/1/19/Go22_29_09.jpg" width=300/></a><var><b>Fig. 1. PCR on pLac_OmpA_omega_</b><br> | ||
+ | 1. Marker<br> | ||
+ | 2. PCR product<br></var> | ||
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</html> | </html> | ||
Revision as of 23:23, 26 October 2008
MutD5 testingPiotrSequencing results: there weren't any mutations in plasmids isolated from MutD5 - maybe this strain is too weak as a mutator. Preparation of pACYC177 + OmpA_omega_Michał K.
Preparation of BioBricksMichał K.
Preparation of vectors for BiobricksPiotrInoculation of bacteria received from iGEM HQs, carrying pSB2K3 and BBa_I739204 (pACYC177 converted into BioBrick vector) plasmids. Fig. 1. PCR on pLac_OmpA_omega_1. Marker 2. PCR product
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