Team:Warsaw/Calendar-Main/3 October 2008

From 2008.igem.org

(Difference between revisions)
Line 22: Line 22:
-
<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+Omp_</h3>
+
<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/Part:BBa_K103006>OmpA-linker (BBa_K103006)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
-
<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmid from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+Omp_).</li>
+
<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmid from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/Part:BBa_K103006>OmpA-linker (BBa_K103006)</a>).</li>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>

Revision as of 00:45, 27 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pSB2K3 + linker_alpha (BBa_K103009)

Piotr

  1. Transformation of TOP10 with ligation pSB2K3 + linker_alpha (BBa_K103009).
  2. Plating on LB with kanamycin.

Preparation of pSB2K3 + linker_omega (BBa_K103013)

Piotr

  1. Transformation of TOP10 with ligation pSB2K3 + linker_omega (BBa_K103013).
  2. Plating on LB with kanamycin.

Preparation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Transformation of TOP10 with ligation pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Plating on LB with kanamycin.

Preparation of pSB1A3+OmpA-linker (BBa_K103006)

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (pSB1A3+OmpA-linker (BBa_K103006)).
  2. Control digest of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of vector for pT7 constructs

Michał K.

  1. Digest of pET15b+OmpA_omega with XbaI (Tango buffer). DNA ends blunting with Klenow fragment (3 hr).
  2. Gel electrophoresis and gel-out of proper band (pET15b+OmpA_omega without XbaI - 6500 bp).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/3_October_2008"