From 2008.igem.org
(Difference between revisions)
|
|
| Line 1: |
Line 1: |
| - | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/11_September_2008 <<Previous] - [https://2008.igem.org/Team:EPF-Lausanne/Notebook Back to Notebook] -
| |
| - | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/22_September_2008 Next>>]
| |
| | | | |
| - | ==Sequencing==
| |
| - |
| |
| - | Before sending the plasmids for sequencing we test our primers by PCR.
| |
| - |
| |
| - | PCR on AB with:
| |
| - |
| |
| - | *pSB1A2 FW, I1466RV
| |
| - | *AB1seqFW, I1466RV
| |
| - | *AB2seqFW,LacImRV
| |
| - | *R0071FW,pSB1A2RV
| |
| - |
| |
| - | PCR on CD with:
| |
| - |
| |
| - | *pSB1A2FW, C0040RV
| |
| - | *CD1FW, C0040RV
| |
| - | *R0040FW, BB3
| |
| - | *R0040FW, pSB1A2RV
| |
| - |
| |
| - | '''Results:'''
| |
| - |
| |
| - | --> image of PCR/gel page 102
| |
| - |
| |
| - | All results were as expected except for AB lane 3, i.e. AB2seqFW, LacImRV
| |
| - |
| |
| - | A new PCR is done for these primers, where the annealing temperature is reduced to 53°C.
| |
| - | This turns out to work.
| |
Revision as of 10:04, 27 October 2008
"
