Team:Warsaw/Calendar-Main/14 October 2008

From 2008.igem.org

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<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmid from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + pT7_omega_link).</li>
<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmid from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + pT7_omega_link).</li>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
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<h3>Preparation of AraC+pBAD+AID</h3>
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<h4>Michał K.</h4>
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<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<ol>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with overnight ligation - <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + AraC+pBAD+AID.</li>
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<li> Tranformants planting on LB with ampicillin. </li>
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</ol>
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Revision as of 11:25, 27 October 2008

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Preparation of linker_alpha (BBa_K103009)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB2K3 + linker_alpha (BBa_K103009).
  2. Tranformants plating on LB with kanamycin.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day - pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Isolation of plasmid from culture inoculated on previous day - pSB1A3 carrying alpha_linker under PT7 (BBa_K103019).

Preparation of pT7_omega_link

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (pSB2K3 + pT7_omega_link).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of AraC+pBAD+AID

Michał K.

Preparation of BioBricks

Michał K.

  1. Transformation of TOP10 with overnight ligation - pSB1A3 + AraC+pBAD+AID.
  2. Tranformants planting on LB with ampicillin.

Piotr

  1. Transformation of TOP10 with overnight ligations.
  2. Tranformants planting on LB with kanamycin - 3kb(?????)+ link_alpha, 3kb(????)_pLac_OmpA_ + alpha2 and RFP(?????) + AraC+pBAD+AID on LB with ampicillin.

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