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EPF-Lausanne/22 September 2008
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(New page: [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/15_September_2008 <<Previous] - [https://2008.igem.org/Team:EPF-Lausanne/Notebook Back to Notebook] - [https://2008.igem.org/wiki/ind...) |
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[https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/29_September_2008 Next>>] | [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/29_September_2008 Next>>] | ||
| - | Sequencing | + | ==Sequencing== |
| + | |||
| + | We got the sequence of AB and CD. | ||
| + | |||
| + | *CD was just as expected. | ||
| + | *There is an error with AB. The part B1 (RBS-LacIm) was not inserted correctly. We blasted the unknown sequence but were not able to find out where it came from. Anyways the test PCR before sequencing shows that it is probably an unwanted interaction, as it only worked when [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/15_September_2008 annealing temperature was reduced]. | ||
| + | |||
| + | We have to redo the PCR of LacIm and the ligation with RBS. | ||
Revision as of 14:52, 27 October 2008
<<Previous - Back to Notebook - Next>>
Sequencing
We got the sequence of AB and CD.
- CD was just as expected.
- There is an error with AB. The part B1 (RBS-LacIm) was not inserted correctly. We blasted the unknown sequence but were not able to find out where it came from. Anyways the test PCR before sequencing shows that it is probably an unwanted interaction, as it only worked when annealing temperature was reduced.
We have to redo the PCR of LacIm and the ligation with RBS.
