Alberta NINT/18 June 2008

From 2008.igem.org

(Difference between revisions)
Sdeane (Talk | contribs)
(New page: lab work (SD): Results: K102000 + I732018.1 - 134 colonies K102000 + I732018.2 - 125 colonies K102000 (negative control) - 10 colon...)
Newer edit →

Revision as of 19:47, 19 June 2008

lab work (SD): 

            Results: K102000 + I732018.1 - 134 colonies
                     K102000 + I732018.2 - 125 colonies
                     K102000 (negative control) - 10 colonies
            Based on sequencing results, 6 colonies from K102000 + I732018.2 plate were inoculated into LB + 
            amp50 culture tubes and incubated at 37 C, shaking, overnight.
            10 ul of K102009 overnight tube from 05/06/08 was inoculated into LB + amp50 culture tube and
            incubated at 37 C, shaking, overnight to create fresh cultures for making a glycerol stock.

            For Wayne: Extracted K102011/H+X from gel using QIA quick gel extraction protocol and a min-elute 
            spin column.  DNA was eluted in 15ul Buffer EB.
Retrieved from "http://2008.igem.org/Alberta_NINT/18_June_2008"