Team:Warsaw/Calendar-Main/18 September 2008
From 2008.igem.org
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2. Digested pSB1A3<br></var> | 2. Digested pSB1A3<br></var> | ||
- | <a name="fig4"><img src="https://static.igem.org/mediawiki/2008/8/85/Go_1909_2008.jpg" width=300/></a><var><b>Fig. 4. PCR to obtain linker_alpha for reisolation from agarose gel | + | <a name="fig4"><img src="https://static.igem.org/mediawiki/2008/8/85/Go_1909_2008.jpg" width=300/></a><var><b>Fig. 4.</b> PCR to obtain linker_alpha for reisolation from agarose gel<br> |
1. Marker<br> | 1. Marker<br> | ||
2. linker_alpha<br></var> | 2. linker_alpha<br></var> |
Revision as of 15:29, 27 October 2008
'Hunter and prey' system tests: Competition testsWeronikaInoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_alpha + induction using 0.25mM iPTG MutD5 testingPiotrInoculation of mutD5 into 100 ml of LB and rendering them chemocompetent. Electroporation with pACYC177+OmpA_Z_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_omega_ΔA. Optimisation of primers for preparation of partsMichał K.
1. Marker 2. 55 °C linker_alpha 3. 60 °C linker_alpha 4. 65 °C linker_alpha 5. 70 °C linker_alpha Preparation of ΔA (BBa_K103003)Michał K.
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.
Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of OmpA-linker (BBa_K103006)Michał K.
Fig. 5. SacI/NdeI digest of pET15b_OmpA_omega 1. Marker 2. deltaA 3. omega 4. ompA_omega Fig. 3. EcoRI/BcuI digest of pSB1A3 1. Marker 2. Digested pSB1A3 Fig. 4. PCR to obtain linker_alpha for reisolation from agarose gel 1. Marker 2. linker_alpha Fig. 5. SacI/NdeI digest of pET15b_OmpA_omega 1. Marker 2. digested pSB1A3
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